Plasmid-enhanced strategy for PCR-mediated mutagenesis with difficult DNA templates.
نویسندگان
چکیده
A.D. Riggs.1999. Chromatin structure analysis by ligation-mediated and terminal transferasemediated polymerase chain reaction. Methods Enzymol. 304:548-571. 14.Pfeifer, G.P., S.D. Steigerwald, P.R. Mueller, B. Wold, and A.D. Riggs. 1989. Genomic sequencing and methylation analysis by ligation mediated PCR. Science 246:810-813. 15.Saluz, H. and J.P. Jost. 1989. A simple highresolution procedure to study DNA methylation and in vivo DNA-protein interactions on a single-copy gene level in higher eukaryotes. Proc. Natl. Acad. Sci. USA 86:2602-2606. 16.Temple, M.D., M.J. Cairns, W.A. Denny, and V. Murray. 1997. Protein-DNA interactions in the human beta-globin locus control region hypersensitive site-2 as revealed by four nitrogen mustards. Nucleic Acids Res. 25:3255-3260. 17.Temple, M.D., M.J. Cairns, A. Kim and V. Murray. 1999. Protein-DNA footprinting of the human epsilon-globin promoter in human intact cells using nitrogen mustard analogues and other DNA-damaging agents. Biochim. Biophys. Acta 1445:245-256.
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عنوان ژورنال:
- BioTechniques
دوره 29 6 شماره
صفحات -
تاریخ انتشار 2000